Sentence examples for using limited cycles of from inspiring English sources

The target library was amplified using limited cycles of PCR.

cDNA libraries were amplified using limited PCR cycles and fragments averaging 150 bp in length were purified.

Secondly, because target specific primer extensions were used in limited cycles and our amplification was mostly driven by a pair of universal primers, we minimized the difference in amplification efficiency and competition among many different amplicons.

Each primer pair amplifies a unique 80-100 base pair cDNA fragment using a limited number of PCR cycles important for maintenance of transcript stoichiometry.

We then proceeded to perform ChIP-seq as described previously [ 8, 9], but with the minor modification of generating libraries using PCR amplification performed in two sequential rounds of limited cycles to help reduce amplification-based background (see Methods).

To distinguish SMN2 (line 89) from SMN2 (line 566), we performed the above PCR using a limited number of 20 cycles and normalized band intensity to that obtained from PCR-based detection of the endogenous, single-copy gene, IL-2 (IL2-F, 5'-ctaggccacagaattgaaagatct-3' and IL2-R, 5'-gtaggtggaaattctagcatcatcc-3') [64].

Then the parameters of the proposed environmental model were calibrated by using a limited number of tests on concrete specimens under freeze thaw-cycles.

Use a limited number of amplification cycles to minimize co-amplification of any maternal DNA.

The residents of Niceland and the bartender from Tapper are animated using a jerky motion that spoofs the limited animation cycles of the sprites of many eight- and sixteen-bit arcade games.

Use limited numbers of miniature tree lights.

A discussion of the related problems of limited cycle life and dendrite formation is presented.