These results are in accordance to observations made using leukaemic cells from CML patients.
Finally, using human leukaemic cell lines, we have extended the concept of FFA2 activation as a tumour-suppressive therapy.
Five leukaemic cell lines were used in the study: 1T cell-derived ALL (Jurkat), 1 CML (K562), 2 AML (HL60 and U937) and 1 CLL (CLL-AAT).
RNA isolated from MCF-7 breast cancer and the ARH-77 leukaemic cell lines was used as positive and negative controls, respectively.
RNA samples prepared from the MCF-7 breast cancer and ARH-77 leukaemic cell lines were used as positive and negative controls, respectively (Stathopoulou et al, 2002).
Metabolic heterogeneity observed in MM cell lines is similar to that reported in leukaemic cell lines, which use glycolysis but also non-glucose bioenergetic pathways such as fatty acid oxidation and/or amino-acid metabolism (Suganuma et al, 2010; Tong et al, 2011).
As detected on leukaemic cell lines Aplidin induced changes in the light-scattering properties by using flow cytometry forward/side light-scatter analysis on ALL cells too, suggesting an induction of apoptosis.
Confocal microscopy was used to visualize the intracellular uptake of the fluorescent methotrexate analogue, fluorescein-MTX (F-MTX), in human leukaemic cell lines and leukaemic blasts.
Two leukaemic cell lines, KG1 and NB4, and 2 bone marrow samples from healthy donors were also included.
Two leukaemic cell lines were included KG1 (a cell line derived from a patient with erythroleukaemia in myeloblastic relapse) and NB4 (a cell line derived from a patient with acute promyelocytic leukaemia).
