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Validations for our S/C ratio estimation was performed with an in vitro biological experiment, where the stromal cell proportions were controlled using known quantity of RNAs as shown in Table 1.
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We present here an advanced protein extraction method using known quantities of proteotypic 13C-labeled peptides to quantify matrix proteins in native and decellularized lung tissues.
Measures of light intensity were compared to a standard curve generated using known quantities of ATP prepared in water, acidified and neutralized as were samples.
A standard curve was performed for all hydroxyproline measurements by using known quantities of hydroxyproline.
In this report, using known input ratios of in vitro transcript we experimentally correlate the quantity of support bound probe to measured expression ratios, in order to validate our quality control threshold for array acceptance.
The gamma well counter was calibrated measuring a known quantity of 18F activity (quantified using a calibrated gamma spectrometer: high purity 30% germanium GR3020 from Canberra Industries, Meriden, CT, USA), thereby obtaining a calibration factor between real and measured activity.
For qPCR, the amount of DNA obtained from a known quantity of plastids was determined using a standard curve of cpDNA amounts.
Thermo-gravimetric analysis (TGA) is a technique used to determine changes in weight of a known quantity of sample in relation to a change in temperature.
Five grams of the sieved sample was mixed with known quantity of distilled water and stirred using a magnetic stirrer.
Serial dilutions of a known quantity of template for each gene were used to measure the copy numbers.
Transcript levels were calculated using the standard curve method (User Bulletin #2, ABI Prism 7700 Sequence Detection System, Applied Biosystems) made from serial dilutions of known quantity of plasmid (10, 10, 10, 10, 102 copies/μl).
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