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Amplicons were detected from all the soil samples artificially infected by using known amounts of FT2 inoculum, whereas none of the primer sets amplified DNA from soils not infected by FT2.
The cytokines concentrations were determined by comparison with standard curves generated using known amounts of the respective mouse recombinant cytokines.
The gene expression data were extrapolated from standard curves generated in parallel using known amounts of input RNA ranging from 200 ng to 3 ng.
A standard curve was generated using known amounts of a plasmid containing the HindIII-N fragment of MHV-68 (containing the gB gene).
Also, we report the results from limit of detection studies, using known amounts of bacterial DNA spiked into aerosol samples to measure the minimal concentration required for detection of virulence elements against a complex background.
A standard curve was prepared using known amounts of the Ca2+ standard included in the kit.
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Cycle threshold detection was converted into number of cDNA contents in the starting material and a standard curve was constructed using the known amounts of cDNA.
Chromatograms were processed using Total Chrome Navigator software and the amounts of identified FAMEs were quantified using calibration curves of known amounts of standards.
An antioxidant standard curve was generated using different concentrations of known amounts of Trolox by ECL measurement and the detection limit for antioxidant capacity was found to be 0.06 mM of Trolox.
The absolute mRNA levels in each sample were calculated according to a standard curve determined using serial dilutions of known amounts of specific templates plotted against the corresponding cycle threshold (CT) values.
Repeatability was assessed in a spiking experiment of soybean in wheat flour (0.0001 100% soybean), using known standards for comparison of the amount of output DNA from different PCR reactions.
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