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Hormone determinations were performed in the same laboratories throughout the years of the study, using kits from the same companies and, frequently, the same technicians.
MRNA capture, cDNA conversion, sizing, and library construction were performed using kits from the Illumina Company and by following the manufacture's recommended procedures.
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Libraries were prepared and sequenced at the USC Epigenome Center using kits from Illumina following the manufacturers' instructions.
DNMT activity in the nuclear extract was determined using kits from Epigentek, following the vendor's instructions.
AMBP and RBP were measured on a Siemens BN II nephelometer using kits from Siemens and The Binding Site, respectively.
Serum calcium and Pi concentrations were determined using kits from Pointe Scientific , Inc(Canton, MI) following the manufacture's instructions (Sigma Diagnostics).
We determined hormones by RIA using kits obtained from the National Hormone and Peptide Program (Torrance, CA, USA).
MLPA analyses were performed using kit P029 from MRC Holland (Amsterdam, Netherlands), following the manufacturer's instructions.
The xanthine oxidase was measured using the using diagnostic kits from Cayman Chemicals according following the procedures mentioned by Marcocci et al.[ 22].
We used labeling kits from the same lots and purchased at the same time, so all labeling reagents were equivalent.
We measured the xanthine oxidase using the using diagnostic kits from Cayman Chemicals.
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