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To sequence the transgenic insertion at the Metrn locus, genomic DNA from tgε26+/+ mice was digested with NcoI, self-ligated, and amplified using inversely oriented PCR primers (Fig. 2H, i-1F, i-1R, i-2F, and i-2R).
We have identified an alternative bioinformatics approach for predicting miRNA targets in human colon cancer and for reverse engineering the miRNA:mRNA network using inversely related mRNA and miRNA joint expression profiles.
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It may sometimes be used inversely to determine the latitude and longitude of the observer, assuming the star positions are accurately known.
Since the average distance of concept in response from the concepts in question is inversely proportional to similarity, thus AvgDist are used inversely in calculating score for users.
If used inversely, the algorithm yields the optimal orientation of a structure on the build platform, allowing further weight reduction while maintaining the mechanical properties.
PaCO2 was used inversely as a surrogate for ventilatory drive 30; decreasing markedly in the first few days at altitude as ventilation increased.
Recently, Sharifzadeh and Thornhill (2012) proposed a modeling approach for control structure selection using an inversely controlled process model, which benefits from significant complexity reductions.
(These stratum-specific estimates were combined over strata using weights inversely proportional to the stratum-specific variances).
Given the study design and sampling strategy, we accounted for variation in sampling probability across facilities using weights inversely proportional to population size, stratification by province and health facility type, and clustering at district and facility levels.
By comparing the ROC analysis results in Table 3 (our method) with Table 5 (conventional method), the advantage of using the inversely transformed VOIs of our method is clear.
Amplicons were visualized by agarose gel electrophoresis in the presence of ethidium bromide and those from the same individual pooled using volumes inversely proportional to reaction yield, in order to establish approximate equimolarity.
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