We first applied our mathematical model to simulate the synergistic inhibition of PHD by succinate and hypoxia, using the intracellular concentrations of αKG and succinate measured in our SDH-loss cell culture experiments (Table 1).
The three genomes lacking glnA or gdhA are all strains of Ureaplasma, which uses high intracellular concentrations of ammonia to generate an electrochemical gradient for driving ATP synthesis [ 21].
The MALDI-triple quadrupole MS assay was used to determine the intracellular concentrations of lopinavir and ritonavir in PBMC obtained from HIV-1 infected children receiving Kaletra (lopinavir + ritonavir) and two NRTIs once daily.
The MALDI-triple quadrupole MS assay was used to investigate the intracellular concentrations of lopinavir and ritonavir in relation to their plasma concentrations in HIV-1 infected children receiving Kaletra, a co-formulation of the HIV protease inhibitors lopinavir and ritonavir (4∶1, w/w), once daily.
ICP-MS was used to quantify the Pd II) or Pt II) content in cells treated with 10 μM Pd-BENSpm, Pd-Spm or Pt-CPENSpm for 72 h and the data were used to calculate the intracellular concentrations of the drugs (Fig. 2).
It has been shown that the dosage of chlorpromazine used in psychiatry results in intracellular concentrations of this drug that are antimicrobial [ 57].
In our opinion, the higher PBMC/plasma ratios when RBCLB is not used is not due to high intracellular concentrations of ritonavir in erythrocytes.
In addition to the cell volume, by using triple-barreled microelectrodes we also measured simultaneously the intracellular concentrations of physiologically relevant ion species as well as the membrane potential.
To test this hypothesis, we assayed the intracellular concentrations of the core NAD+ metabolome using a recently developed LC-MS assay [16], [17].
Alternatively, high intracellular concentrations of reduced glutathione [ 21] could be used elute the GST-protein from the glutathione-containing material.
