Sentence examples for using individual primer sets from inspiring English sources

Table 1 indicates the CoV genera identified by using individual primer sets.

Changes in individual genes were confirmed using individual PCR primer sets (SABiosciences; Table 1).

Part of the mitochondrial COI gene was amplified using various primer combinations (as individual primer sets did not work for all species) (Table 2).

Sensitivity of the individual primer sets used in the RT-PCR assays was tested by spiking negative control lung tissues from chicken and pig with virus inoculum before homogenization, titrating out the homogenate, and running the RT-PCRs in parallel on the same RNA extracts.

All individual primer sets for genes of interest were designed using NCBI primer design on exons junction when possible.

Sensitivity of the RT-PCR employing the individual primer sets is illustrated in Table 1, as determined by using negative control lung tissues spiked with SARS-CoV.

coli isolates was tested with individual primer sets (simplex assay) and all primer sets including stx1, stx2, and uidA(multiplex assay).

Forward and reverse primers in individual primer sets are labeled with distinct molecular weight tags.

To test the specificity of the primers pairs, PCR was carried out separately with the individual primers sets using pure gDNA of P. cajani in 50 μL reaction mixture containing: 5 μL of 10 X Taq polymerase buffer, 1.5 μL 50 mM MgCl2, 1 μL of 10 mM each dNTP, 1 μM of each primer, 1 μL of 5u/1 μL Taq polymerase (Invitrogen, USA), about 100 ng of gDNA and H2O up to 50 μL.

PCR reactions were performed using individual forward primer and the P3 reverse primer.

In this case, we had to use individual sets of MF a 1 primers because of the substantial polymorphism observed at the 5´ and 3´ end of this gene between Cg and Cn, and within Cn vatietie s.