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As the plasmid copy number was estimated to be 3, the retrieval plasmid was selected using increased numbers of marker genes derived from the retrieved DNA.
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A 2017 study concluded that women are playing an increasingly significant role in terrorism, and Islamic State has been using increasing numbers of women to spearhead attacks.
Non-Hindi speakers, however, are pulling the language in another direction by using increasing numbers of English words and phrases and by simplifying the complex rules of subject-verb agreement found in standard Hindi.
And as businesses look to become more agile, using increasing numbers of freelancers seems a clear path they are taking.
And carmakers such as Volvo and Volkswagen are using increasing numbers of white LEDs for interior lighting.
It also found that U.S. consumers — far from being bored of apps, as third mobile ecosystem platform contenders sometimes try to argue — are actually using increasing numbers of apps per day.
The target sequence was amplified using increasing numbers of cycles of 95°C for 15 s, 56°C for 30 s, 72°C for 15 s as described previously [26], [42].
The primer set for COX2, FN1 and VEGF was amplified using increasing numbers of cycles of 95°C for 15 sec, 58°C for 30 sec, 72°C for 10 sec, and a final extension of 72°C for 1 minute.
The primer set for CD73 was amplified using increasing numbers of cycles of 95°C for 15 sec, 60°C for 30 sec, 72°C for 10sec, and a final extension of 72°C for 1 minute.
The number of correctly classified animals (infected versus non-infected) at day five and day 19 post infection using increasing numbers of SELDI-TOF-MS protein components.
The range of thresholds was used to demonstrate changes in the overlap zones created using increasing numbers of intersecting daily activity areas.
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