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After thawing on ice, the cells were washed twice with buffer without Triton X-100 and the OD600 was adjusted to 1 before using in the assay.
Sera were diluted 1 80 in IgM assay diluent (IgG assay diluents lacking goat serum and KCl) and pre-incubated with Serion Rf-Absorbent as recommended by the manufacturer (Virion/Serion GmbH, Würzburg, Germany) to remove IgG antibodies before using in the assay.
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By contrast, fullerenes and activated carbon used in the assay were largely ineffective.
The BSA protein was used in the assay as a negative control.
Numbers indicate, number of times the same beads were used in the assay.
All chemicals used in the assay were of at least reagent grade.
The half-saturated solution of β-carotene in acetone used in the assay was prepared as follows.
The substrates D-ribose-5-phosphate (R5P) and d-fructose-6-phosphate (F6P) were used in the assay.
Labeled viruses could be used in the assay of virus-specific cytotoxicity, which might reflect the natural process more closely.
Altering the amount of antagonist used in the assay can alter the dose ratio.
The resulting solution was filtered and used in the assay.
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