Sentence examples for using immortalized cell lines from inspiring English sources

It is of particular importance for assuring reliable and useful results when using immortalized cell lines to guarantee cell authenticity, since it is estimated that 15%to20%0% of the cells used in experiments are not what they are supposed to be [ 156].

Given the intrinsic limitations of the classical rotavirus models using immortalized cell lines infected with laboratory-adapted strains in two dimensional cultures, our study aimed to model infection and antiviral therapy of both experimental and patient-derived rotavirus strains using three dimensional cultures of primary intestinal organoids.

Most screens have therefore been performed using immortalized cell lines overexpressing CFTR mutants and halide indicators [9].

Our analysis was limited to macrophage-like cells and we acknowledge the limitations inherent in using immortalized cell lines in vitro.

The host cell's ability to respond to these diverse signals is clearly important, yet may not be evident when using immortalized cell lines due to the various mutations or altered signaling pathways involved in the immortalization process.

Because the original observations were made using immortalized cell lines, it is important to verify that primary strains display the same difference in SAC stability.

Some reported in vitro models use primary cells, while others use immortalized cell lines.

For the CFTR-expressing cell model, we used immortalized cell lines of murine renal distal convoluted tubules (DCT) or proximal convoluted tubules (PCT) (Barriere et al., 2003; Milosavljevic et al., 2010; Peyronnet et al., 2012).

Previous human studies investigating the role of fibroblasts in DFUs have primarily used immortalized cell lines following extensive passaging isolated from tissues that are not from the dermal foot region, which are less predictive of in vivo patient outcomes.

This system may hold great potential for reducing drug development costs and may provide significant advantages over current industry standard assays that use immortalized cell lines or animal models.

Molecular consequences of several HSPB1 missense mutants have previously been addressed in overexpression studies using either immortalized cell lines or primary neuronal cells from rodents [30], [31], [33], [56].