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Image processing of the fluorescent images was performed using ImageJ software to obtain the changes in fluorescent intensity across the gel at each time point.
Micrographs obtained via SEM were examined using ImageJ software to quantify the adhesion of S. aureus and E. coli O157 H7 to substrates covered with varying functional groups.
Unprocessed original scans of western blots are shown in Supplementary Fig. 7. Relative quantification was performed using ImageJ software to determine the STING/beta-actin ratio for each sample.
Consequently, in this paper, we consider the effective size and aspect ratios of digital images for shape analysis using ImageJ software, to be 100 to 1024 pixels and 10 1 to 10 10, respectively.
Images were analyzed using ImageJ software to count the number of invaded particles containing ≥1 cell (N), the total area invaded (A), and the depth of invasion (D).
The images were analyzed using ImageJ software to calculate the NP coverage on the vWF substrate.
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To determine P2 glomerular volume, we used ImageJ software to calculate cross-sectional areas of a particular glomerulus in serial sections.
To quantify nuclear Smad3 and Smad4 accumulation over time in normal HKc and all four HPV16 immortalized lines, we used ImageJ software to analyze the immunofluorescence images.
We used ImageJ software to measure the representative cell domain and cell body surface area in each age group (number of cells=15 in both controls and 3xTG-AD animals).
The intensity of individual bands from western blots was quantified using ImageJ software, normalized to GAPDH, and then shown relative to the control group value.
Band intensities were quantified using ImageJ software, normalized to untreated samples and expressed as arbitrary units relative to controls.
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