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For analysis of size distribution from transmission electron micrographs, at least 300 particles were analyzed using imageJ program to calculate the actual size and size distribution of Ag NPs formed by all above-mentioned recipes.
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Cell rounding was evaluated using ImageJ program.
Cells were counted using ImageJ program (NIH).
Increased levels of p-p53Δ and p-Mdm2 were quantified using ImageJ program (Table 1).
Quantification of the signals on the membranes was carried out by using ImageJ program.
Peptidase activity was estimated by the intensity of the bands using ImageJ program (NIH).
PCR products were separated on agarose gels, stained with SafeView probe (NBS Biologicals), visualized using gel documentation system (Hoefer) and analysed using ImageJ program.
In this case, at least 50 NPs were analyzed using ImageJ program and the average value and its standard deviation were calculated, respectively.
To quantify cell migration, the uncovered area where no cells were present was measured by using ImageJ program, and the ratio of uncovered area between untreated control and treated groups was obtained.
For statistical analysis on the percentage pathlength in the center region, we used ImageJ program (developed at the U. S. National Institutes of Health, and available on the Internet at http://rsb.info.nih.gov/ij/), Mice were allowed to explore freely while data was collected for 30 min. In the light and dark test, the square arena was divided into light and dark compartments.
In this study, we developed a cell-collecting kit for the use of BMVC staining and combined ImageJ program to digitize BMVC fluorescence intensity, hereafter named BMVC test.
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