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The high-resolution TEM image in c was Fourier-filtered using ImageJ package to reduce the background noise.
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The densitometry of bands was performed using ImageJ package (http://imagej.nih.gov/ij/).
If applicable, band densities corresponding to free, singly and doubly bound nucleosomes were quantified using ImageJ software package and subsequently fitted together to a 2 1 binding model using in-house written MatLAB routine (MATLAB version 7.13.0, The MathWorks Inc., Natick, MA).
Western blot quantification was performed using ImageJ software package (NIH, Bethesda, MD, USA) according to the developer's instructions.
Endpoint image analysis of Gene-Z cards consisted of obtaining the signal intensities of the second (2 min) and last image (60 min), which were extracted from 16-bit images using ImageJ software package (http://rsbweb.nih.gov/ij/).
Eye area was measured using ImageJ blind to genotype.
Relative expression was calculated using ImageJ to quantitate band intensity.
The intensity of ethidium-bromide luminescence and protein expression in Western Blot images was quantified densitometrically using ImageJ image-processing software package (ImageJ: National Institutes of Health, Bethesda, MD, USA), as abovementioned, and normalized in respect to the corresponding fragment concentration of the ubiquitously expressed genes PBGD and β-actin.
Induced sputum samples were processed and analysed using ImageJ and Image SXM software packages.
All images were processed using ImageJ (NIH) and the LOCI Bioformats Java package.
The existing and proposed methods for extracting features were implemented by using ImageJ [ 53], an image processing package based on the JAVA programming language.
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