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Eye area was measured using ImageJ blind to genotype.
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Quantification of bacterial cell staining was performed using ImageJ software according to its standard manual.
Images were acquired using ImageJ software, converted to TIFF images and arranged using Adobe Photoshop 7.0.
Relative expression was calculated using ImageJ to quantitate band intensity.
Protein expression levels were quantified using densitometric analysis using ImageJ and normalized to tubulin expression.
The intracellular MOR vesicles were counted using ImageJ software by an investigator blind to treatment.
Postcollection processing was performed using ImageJ and applied uniformly to all paired images.
This distance was measured in pixels using ImageJ and then normalized to littermate controls.
Within each cyst, four random regions of interest were selected capturing the fluorescence profile from the basal to the apical membrane using ImageJ (V.1.45, U.S. National Institutes of Health) with the investigator being blinded to the experimental condition.
Quantitative image analysis was performed blinded using ImageJ software (rsb.info.nih.gov/ij/).
Densitometry was performed using ImageJ software (NIH) and normalized to GAPDH levels of the representative gel.
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