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The first was based upon Agilent Technologies DNA microarrays taken from Prat et al., with 42 new additional metastasis samples profiled here using identical protocols as previously described [ 6, 19, 37].
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Patient and control samples were collected in a random order as they became available and at the same location using identical protocols, instruments and study personnel.
The beverage and bar were assessed in 2 separate studies using identical protocols.
All individual and combined analyses were completed using identical protocols.
For this study, we collected and processed specimens in 2005 and 2009 using identical protocols.
By accepting these logistical limitations, patient and control samples could be collected as they became available at the same location, generally in a random pattern and in large numbers, using identical protocols, instruments and study personnel.
All radiographs were taken using identical protocol with non-digital technique by the same technicians over the study period.
Both studies used identical protocols and an integrated analysis of all data was prospectively planned.
All LLS sites use identical protocols and are subject to a quality control program (18).
Exposure to BPS, egg count and habituation measurements were carried out using essentially identical protocols as described for BPA above.
We constructed the Illumina libraries using the identical protocol as ref. [ 6] which used unmethylated oligonucleotides, followed by amplification of only bisulfite converted oligonucleotides, to ensure that only fully bisulfite converted DNA is incorporated into the libraries (see Methods).
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