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Specificity was controlled using identical concentration of an isotype-matched antibody (IgG2a; 21335021; Immunotools).
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Using identical concentrations of cell-function inhibitors used previously to study endothelial cell invasion [17], entry of S. aureus into HaCat cells was also found to involve PI3 and Src-kinases as well as actin rearrangement.
Each siRNA was added to 10 nM and compared with controls transfected using identical concentrations of control siRNA mixture.
Using a haemolytic assay we were able to detect AP-mediated lysis using sera from MASP1/3 –/– mice, although this was lower than that seen in wild-type mice using identical concentrations of sera.
We used identical concentrations of F16 and L19 antibodies (Pedretti et al, 2009).
cDNA libraries for PolyRibo-3′ RNA-sequencing was performed using identical concentrations from each of the RNA sample fractions collected after polysome fractionation that was performed as indicated above.
Negative controls were prepared by using the identical concentration of mouse immunoglobulin IgG1 (Dako).
The inhibitory effect of ketoconazole was tested by using identical protein concentration in all tissues studied because ketoconazole is lipophilic, and the unbound concentration of the drug may be influenced by total protein content in the assay mixture.
Briefly, sections were processed in parallel using identical antibody concentrations.
The reported peak areas and peak heights were compared to those obtained injecting diluted standard solutions of the same perchlorate concentrations using identical conditions.
Absolute dimethyl sulfoxide (DMSO; Calbiochem) was used as solvent to make stock solutions, which were typically diluted 1 1000 to obtain working dilutions; identical concentration of DMSO was used as vehicle control.
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