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Gene Ontology functions were assigned using GOblet.
GO classifications were obtained using Goblet and Interpro information as well as AutoFACT.
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While our 3-D HT-29 cells did exhibit mucus production (a hallmark of goblet cells), we were unable to definitively identify goblet cells in the 3-D HT-29 cells by immunofluorescence microscopy using a goblet cell marker antibody [32], [67].
Of the 5040 functionally annotated and domain name-containing sequences, Gene Ontology (GO) classifications were found for 3878 using AutoFACT, Goblet and InterPro.
Gene Ontology (GO) annotation was performed using the GOblet software package [ 44] and a GO term associated to each sequence showing a significant similarity hit by BLASTX against SWALL search was defined.
At least 20 intact villi were used for goblet cell index for each rat jejunum (400X).
You do not need to use a goblet, but the glass you use should have a narrow enough mouth so that it can hold the napkin in the same general position you held the napkin in before inserting it into the glass.
Colored glass vases (top right), designed by Mr. Hoffmann in 1915, can also be used as goblets or dessert bowls.
To top of the shirt, Cage is rocking a sweet cowboy hat and leather pants, whilst drinking from a goblet and using a cane, because why not.
These 3-D reconstructed images allowed easier differentiation of epithelial gaps from goblet cells, using criteria previously developed by Kiesslich et al. Adequately imaged villi, defined as en face villi images showing 75% of villi surfaces on the CLE images, were used for counting of epithelial cells and gaps.
Segments underwent two mean inclusions: (1) in paraffin to obtain semi-seriated sections of 7 μm thickness each and subjected to hematoxilin and eosin (HE) staining and (2) in historesin (LKB, Technovit 7100) to obtain semi-seriated sections of 7 μm thickness each to histochemically identify glycoproteins present in intestinal goblet cells using the PAS method.
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