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No lag phase was observed when using glucose concentration of 4 g/l.
a c and d f represent data obtained from using glucose concentration of 10 and 4 g/l, respectively.
Since there was no lag phase when using glucose concentration of 4 g/l, the bar chart results were omitted.
The results shows that when using glucose concentration of 4 g/l, no lag phase was observed (Fig. 2a) while with 10 g/l the lag phase lasted for about 190 min (Fig. 2b).
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A standard curve was constructed using glucose concentrations of 0.625-10 mM from which eventual IU values were calculated.
For a biochemical definition, surgeons, defining spontaneous pathological hypoglycaemia requiring investigation and treatment, and forensic pathologists have used glucose concentrations of < 2.2 mmol/l, to avoid defining healthy people as hypoglycaemic [ 5, 6].
Figure 4a c shows the initial comparative results of ethanol production between free and immobilized cells using higher glucose concentration of 200 g/l and stirrer speed 500 rpm.
RR was calculated using the glucose concentration of the arterial blood sample divided by the mean concentration of the first 2 samples from microdialysis.
There are also some concerns with using glucose dialysate concentrations of 200 mg/dL because hyperglycaemia may be a proinflammatory stimulus [ 94– 94].
We used various concentrations of glucose ranging from 7.8 mmol/l (equivalent to FBS of 140 mg/dl) to 19.5 mmol/l (equivalent to FBS of 345 mg/dl) to simulate hyperglycemia because these are more or less similar to the conditions in diabetic patients, whereas previous studies had used a higher glucose concentration of 33 mmol/l [ 30- 33].
However, In the previous study, they used a blood glucose concentration of 200 mg/dL (11.1 mmol/L) as the threshold for differentiating high glucose concentrations from normal glucose concentrations, and concluded that hyperglycemia within 24 h of PICU admission was not independently associated with increased mortality.
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