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Gene prediction was performed using Glimmer v. 3.02, and functions of the gene products were annotated by BLAST + using NCBI-nr protein database.
Gene prediction was performed using Glimmer v. 3.02 (Delcher et al. 2007), and functions of the gene products were annotated by BLAST+ (Camacho et al. 2009) using NCBI-nr protein database (Coordinators 2017).
Automated gene prediction was done using Glimmer version 3.02 [77].
Putative coding sequences (CDSs) were identified using glimmer [17].
An initial set of ORFs that likely encode proteins was identified using GLIMMER [34].
Coding regions were predicted using Glimmer 2 [29] and annotation was performed using GAMOLA [30].
Open reading frames (ORFs) likely to encode proteins (CDSs) were identified by using GLIMMER.
An initial set of predicted protein-coding regions was identified using GLIMMER [91], [92].
After assembly, the plasmids were manually quality controlled and open reading frames (ORFs) were predicted using Glimmer 3.02 [53.02
sORFs (25 100 amino acids) were predicted using Glimmer, RBSfinder, and GeneMark.hmm, using default parameters [69], [70], [71].
Coding sequences were predicted using Glimmer in conjunction with ELPH for selection of optimal start sites [123].
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