Sentence examples for using gateway from inspiring English sources

All proteins were cloned into this vector using gateway cloning per the manufacturers instructions.

Vectors were prepared using GATEWAY recombination technology following the manufacturer's instructions (Invitrogen).

For the Y2H assay, vectors were created using gateway technology (Invitrogen).

Additionally, the promoter region was inserted into the destination vector pMDC206 [ 36], using gateway technology (Invitrogen).

Human LXRα, LXRβ and their mutants were cloned into pDEST retrovirus vector using gateway technology (Invitrogen, Carlsbad, CA).

The resulting FKBP12-Abl kinase domain fusion construct was shuttled to pcDNA3.1-N-V5-TEV (Invitrogen) using gateway cloning.

SHARP-1 and -2, NPAS2 and BMAL1 encoding constructs were assembled using Gateway-mediated recombination as described [41].

Using Gateway-compatible primers the promoter::coding sequence fusions described above were amplified.

The 35S NTAP AtCTF7∆B construct was generated using Gateway-compatible binary vectors containing the NTAP tag ((NTAPi) [ 30]; a gift from Dr. Qinn Li lab, Miami Univeristy).

Proteins interacting with the TaWRKY53 promoter were identified in yeast one-hybrid assays using Gateway-based DNA bait and protein expression prey vectors (Deplancke et al. 2004).

Gateway cloning was performed using Gateway BP and LR Clonase® Mix II from Invitrogen.