Sentence examples for using gag from inspiring English sources

Individual GAGs were estimated using GAG degrading enzymes and nitrous acid treatment as described by Breen et al. [ 23].

Finally, the cells were harvested and the level of expression of Gag was evaluated by Western blot, using Gag antibodies.

Monocyte-derived macrophages from 393 blood donors were infected with HIV-1 and viral replication was determined using Gag p24 antigen levels.

We constructed pJCT, a plasmid containing the LTR-LTR junction, as follows: The HIV-1 LTR-LTR junction was amplified from HIV-1-infected CEM cells using gag 5'-gaattcgcgcttcagcaagccgagtc and env 5'-gaattcacccaaaaggtcagtgtggagtcc primers.

In Additional file 2 AF2B, we show diverse sequence comparisons using gag and pol ORFs of DrFV-1 as queries against the search of taxonomic HMMs available at the Gypsy Database (GyDB) [ 26].

We previously developed a technology termed GAG-binding enhanced transduction (GET) to efficiently deliver a variety of cargoes intracellularly using GAG-binding domains to promote cell targeting, and cell penetrating peptides (CPPs) to allow cell entry.

It has been demonstrated that the estimate of Gag distribution obtained using Gag-CEST at 7 T exhibits an excellent correlation with that obtained using MR-based sodium imaging [64], which is considered to be the gold standard for in vivo Gag imaging.

We interpret these findings as MHV-68 using GAGs as a gp150-dependent entry signal, rather than a significant physical attachment.

6) The rationale for using Gag-GFP in some of the experiments is unclear.

Purified GAGs were depolymerised using GAG-specific enzymes (Seikagaku Corporation, Tokyo, Japan).

Using gag-pol polyprotein sequences available from public databases, we selected amino acid sequences between reverse transcriptase motifs I to VII to build the Neighbor-Joining tree based on the Poisson distance model.