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Since TWEAK-Tg mice were generated in B6D2F1 background, these mice were crossed with C57BL/6 mice for 7 generations before using for this study.
Our in vivo data are limited to the mouse-recipient strain, which we have been using for this study (Nod/Scid mice), and we are aware that the use of different mouse recipients can have an impact on the tumour-initiating capability of injected cells (Quintana et al, 2008).
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No new mice were used for this study.
Sprague-Dawley rats were used for this study.
Two clones were used for this study termed MT6 and MT20.
APPsw/PS1ΔE9 transgenic mice were used for this study, as previously reported56.
Typical imaging rates used for this study were 0.2 or 0.15 s per frame.
Both male and female adult mice (3 5 months old) were used for this study.
A total of 139 patient samples were used for this study.
Figure 1 outlined the overall approaches that we used for this study.
Both males and females (8 14 weeks of age; genders housed separately) were used for this study.
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