Sentence examples for using final dilutions of from inspiring English sources

Staining of cells was performed as described [14] using final dilutions of the mAb as indicated by the manufacturer.

All primary and secondary antibodies were used at final dilutions of 1/1000 and 1/2000, respectively.

CD11b and CD45 antibodies were fluorochrome-conjugated with PacificBlue and FITC respectively, and used at final dilutions of 1 10.

Mouse monoclonal anti-Rac1 antibody (ab33186, Abcam) was used at final dilution of 1 : 750 as suggested by the manufacturer.

The final dilutions of the antibodies used were the following: anti-PDF antibody 1 500 (C7-DSHB), anti-TIM antibody 1 2000 (Myers et al. 1996), and anti-PER antibody 1 1000 (from J. Hall, Brandeis University, Walthman, MA).

Pansorbin is a suspension of heat-killed and formalin-hardened S. aureus Cowan I cells and was used at a final dilution of 1 2500 (v:v); ConA was used at a final concentration of 10 μg/mL in the cell culture.

LPLUNC1 antibodies were used at a final dilution of 1 100 (LPLUNC1A) or 1 600 (LPLUNC1B) following antigen retrieval using tri-sodium citrate in a microwave for 8 min (Bingle et al. 2007).

The inhibition of caspase-3 and -7 was studied by using the Z-DEV-FMK inhibitor (ALX-260-141-R100) from Enzo and was used at a final dilution of 10  μM on whole CD4+ cells for 2 h.

Antibodies were used at a final dilution of 1 : 2000, except the EPAS 1 and β-actin antibodies which were used at 1 : 1500 and 1 : 5000.

Neutralisation of delNS1 virus was performed using sheep anti-serum reagent to hemagglutinin (HA) of A/PR/8/34 virus (03/242, NIBSC, Hertfordshire, UK) used at a final dilution of 1 40.

Immunoreactive CGRP was quantitated using a rabbit antiserum R-84299) ragainstgainsynthetictic rat CGRP conjugated to bovine serum albumin (BSA) and used at a final dilution of 1 37,500.