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Using fibroblast cell lines derived from CEDNIK patients, we show that SNAP29 mediates endocytic recycling of transferrin and β1-integrin.
Chromatin immunoprecipitation (ChIP) with anti-CTCF using fibroblast cell lines from two normal individuals showed considerable enrichment of CTCF in the 5'UTR of the FXN gene in vivo, which was comparable to that seen at the MYC locus, used as a positive control (Fig. 1C).
In vitro studies examined inhibition of Chlamydia proliferation using fibroblast cell lines as targets and phase contrast microscopy.
More recently, using fibroblast cell lines from affected as well as unaffected family members, we have been able to undertake more in-depth analysis into the consequences of non-polyadenylation of mt-mRNAs.
In order to determine the performance of some of the existing microarrays, Affymetrix Porcine, Affymetrix Human U133+2.0, and the U.S. Pig Genome Coordination Program spotted glass oligonucleotide microarrays were compared for their reproducibility, coverage, platform independent and dependent sensitivity using fibroblast cell lines derived from control and parthenogenic porcine embryos.
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In-vitro cytotoxicity and biocompatibility behaviour of ZrO2 film were investigated using fibroblast cell line, which exhibited high degree of cell viability and proliferation rate rather than bare 316L SS substrate.
The toxicity of NO/THCPSi NPs, glucose/THCPSi NPs, and THCPSi NPs at different concentrations (0.05 to 0.2 mg/mL) over 48 h was evaluated using the NIH/3T3 cell line, which is one of the most commonly used fibroblast cell lines and often used as a model for skin cells.
We used fibroblast cell lines that stably express human 15LO or LacZ (as a negative control) [37] to test whether our 15LO-expressing cells (15LO cells) produce excess pro-inflammatory proteins compared to LacZ-expressing cellsoLacZlls (Lacellslls).
In order to provide a plausible answer, they used fibroblast cell lines and showed that TGF- β1, a profibrotic cytokine which is overexpressed in IPF, inhibits autophagy.
To investigate this hypothesis, we used fibroblast cell lines derived from fibronectin-/-, integrin-β1-/ (so called GD25 cells), FAK-/ and c -src-/-, c -fyn-/, and c -fyn-/- (SYF) triple knockout mice [ 36, 38, 41, 42], which completely lack expression of the respective genes.
We chose NIH-3T3 cells as the background because they are one of the most commonly used fibroblast cell lines, are very easy to culture, express wild type p53 protein, and have been extensively studied for DNA damage, showing sensitivity to different types of genotoxic agents.
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