Sentence examples for using established protocols as from inspiring English sources

RNA was isolated using established protocols as described previously [ 7].

Fixation, staining, and microscopy of frozen cryosections (5  μm) of thymi from young and aged mice were performed using established protocols as previously described.

Double-stranded cDNA is synthesized using established protocols while the immobilized oligonucleotide serves as primer for the first strand.

Derivation of hESC lines from blastocysts following PGD was carried out using established protocols and as we previously described (Frumkin et al., 2010).

Microarray analysis was done using established protocols and details are provided as supplementary data.

Laser scanning confocal microscopy was used to monitor live cell accumulation of peptide as well as identify subcellular localization of peptide accumulation using established protocols.

Renal markers, including creatinine, blood urea nitrogen, antioxidant enzymes as well as markers for lipid peroxidation were determined using established protocols.

CHC knock down was confirmed by Western blot using monoclonal antibody against CHC (TD.1, Santa Cruz-12734) as primary and goat anti-mouse-HRP secondary antibody using established protocols.

DNA was extracted using established protocols.

Western blots were performed using established protocols.

RNA extractions were performed differently using established protocols specific to individual laboratories.