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The same procedure was repeated with the bottom layer by using equivalent volume of other solvents like dichloromethane, ethyl acetate, n-butanol, ethanol, and water to obtain DCMPA, EAPA, BPA, EPA, and APA, respectively.
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Control mice were sham sensitised using an equivalent volume of PBS/Alum.
PCR reactions were carried out using equivalent amount of cDNA and one unit of GoTaq DNA polymerase (Promega, Madison, WI) in a final volume of 25 µl.
Where inhibitors were utilized, they were dissolved in DMSO and used at the indicated concentrations with an equivalent volume of DMSO used as a control.
Data were converted into an equivalent volume of internalized fluid using a calibration curve [ 42].
Both the dsRNA of GFP and an equivalent volume of buffer were used as controls.
The well-documented proteasomal inhibitor MG132 was used as a positive control, and an equivalent volume of solvent as a negative control.
Briefly, LAM (20 mg/kg) and ETV (0.1 mg/kg) were given twice per day by oral gavage administration in a medication course lasting 12 days, and the equivalent volume of saline was used as a control.
Injection of equivalent volume of gfp dsRNA was used as control.
An ozone generator (Multiossigen, Gorle, Bergamo, Italy) was used to erogate the O2/O3 gas mixture, composed of an equivalent volume of ozone-oxygen (1 1 volume relationship).
An equivalent volume of distilled water instead of the sample was used as the blank.
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