Sentence examples for using equivalent quantities of from inspiring English sources

The total four RNA from each sample was then pooled to one, using equivalent quantities of each sample.

Using equivalent quantities of DNA extracted from MTB-infected samples as templates at various dilutions, the detection limits for LAMP and PCR were both at 10−4 dilution and 3(b)).

We chose the Hmbs gene as control housekeeping gene because it showed low variability in Ct between all testis stages when equivalent quantities of cDNA were used as template.

Equivalent quantities of RNA were reverse transcribed using iScript kit (BioRad) and real-time PCR was performed in the ICycler system (BioRad), using iQ SYBR green supermix (BioRad) and gene specific primers.

In order to reduce the variability in the concentration of the initial RNA populations across samples and to use equivalent RNA quantities to the ones used for array hybridization, 12 ng of the total RNA of each sample was used for cDNA synthesis using oligo (dt)25 and random primer as described elsewhere [ 58].

For the hemizygous experiments and the competition assay, equivalent quantities of mock-transfected cells seeded in 24-well plates were used to subtract the background fluorescence.

Conversely, equivalent quantities of cells transfected with 0.5 ug atxn7-100-A-DsRED atxn7-100-A-DsRED atxn7-100-A-DsRED atxn7-100-A-DsRED +1.5st reading through the green channel.

Secondly because the overlap cannot be completely removed, equivalent quantities of cells transfected with 0.5 ug atxn7-10-G-eGFP +1.5 µg of pTZU6+1 were used as the background values whilst reading through the red channel.

Equivalent quantities of protein (20 μg) were separated by SDS-polyacrylamide gel electrophoresis and transferred onto polyvinylidene fluoride membranes (Millipore) using established procedures.

In addition, 2Me-diplopterol produces 10× higher ion counts than equivalent quantities of 2Me-BHT.

The controls were set up with equivalent quantities of DMSO as control.