Sentence examples for using equivalent amounts of from inspiring English sources

Instead, we performed our experiments using equivalent amounts of total RNA template.

Thus, to compare the relative abundance of mRNA transcripts in ESCs with ESMVs, we relied on using equivalent amounts of ESC and ESMV total RNA in each experiment.

Using equivalent amounts of starting total RNA template, we detected a miR-16 signal only with the RNA samples containing small RNAs (not shown).

QD delivery enhances T cell priming, leading to a 5 20 fold shift in the dose required to stimulate proliferation and a strong enhancement of IFN-γ production, compared to a standard immunization using equivalent amounts of free antigen (ovalbumin).

The analysis has been performed using equivalent amounts of proteins for each given fraction and it is not informative on relative distribution of the proteins analysed in the different cellular compartments.

Positive control experiments were conducted with wild-type strains using equivalent amounts of isolated mRNA extracted from eIF4Ep immunoprecipitated fractions.

PCR reactions were carried out using equivalent amount of cDNA and one unit of GoTaq DNA polymerase (Promega, Madison, WI) in a final volume of 25 µl.

PCR reactions were carried out in a GeneAmp PCR System 9700 (Applied Biosystems, Carlsbad, CA) using equivalent amount of cDNA and one unit of Titanium Taq DNA polymerase (Clontech, Palo Alto, CA) in a final volume of 25 µl.

Using equivalent amount of total RNA (300 μg) requires a substantial RNA source and is unrealistic in most experiments.

Regardless of the type of antibodies used, equivalent amounts of co-precipitation of Magmas with DnaJC19 (yPam18 ortholog) supports the existence of a similar subcomplex in humans (Fig.  5B and C).

This hypothesis is relevant in cases where we use equivalent amount of RNAs, cDNAs or sequence templates as for usual RNA-seq, but it would be inappropriate for single-cell transcriptome sequencing where the underlying biological heterogeneity may include heterogeneity of the total transcript count between cells.