Sentence examples for using equivalent amount of from inspiring English sources

PCR reactions were carried out using equivalent amount of cDNA and one unit of GoTaq DNA polymerase (Promega, Madison, WI) in a final volume of 25 µl.

PCR reactions were carried out in a GeneAmp PCR System 9700 (Applied Biosystems, Carlsbad, CA) using equivalent amount of cDNA and one unit of Titanium Taq DNA polymerase (Clontech, Palo Alto, CA) in a final volume of 25 µl.

Using equivalent amount of total RNA (300 μg) requires a substantial RNA source and is unrealistic in most experiments.

Instead, we performed our experiments using equivalent amounts of total RNA template.

Thus, to compare the relative abundance of mRNA transcripts in ESCs with ESMVs, we relied on using equivalent amounts of ESC and ESMV total RNA in each experiment.

Using equivalent amounts of starting total RNA template, we detected a miR-16 signal only with the RNA samples containing small RNAs (not shown).

The analysis has been performed using equivalent amounts of proteins for each given fraction and it is not informative on relative distribution of the proteins analysed in the different cellular compartments.

QD delivery enhances T cell priming, leading to a 5 20 fold shift in the dose required to stimulate proliferation and a strong enhancement of IFN-γ production, compared to a standard immunization using equivalent amounts of free antigen (ovalbumin).

Approximately 50 μg of total proteins was loaded on 12% SDS PAGE gel using equivalent amounts of protein.

Positive control experiments were conducted with wild-type strains using equivalent amounts of isolated mRNA extracted from eIF4Ep immunoprecipitated fractions.

Using equivalent amounts of DNA from individual plants, samples were pooled fourfold and organized into 96-well format.