Sentence examples for using equal volumes of from inspiring English sources

Immunoprecipitations were performed using equal volumes of the precleared lysate incubated with EZ view Red Anti-FLAG M2 Affinity gel (Sigma) or Protein A/G PLUS agarose beads (Santa Cruz) and rabbit anti-Sox2 (WRAB-Sox2, Seven Hills Bioreagents, 3 µl) or normal rabbit IgG (1.5 µg; Santa Cruz).

Libraries were diluted to equal molarity and finally pooled by using equal volumes of each library.

DNA was then successfully isolated from all 56 patient samples using equal volumes of saliva.

Three rounds of organic extraction were performed using equal volumes of phenol, 1 1 mixture of phenol/chloroform, and chloroform.

Screeing was conducted in Compact Jr. (Emerald biosystems) sitting drop vapor diffusion plates at 20 °C using equal volumes of protein and crystallization solution.

Gel blocks were made by using equal volumes of 2% low-melting-point agar (BioRad, USA) and a bacterial suspension of 9 × 10 cells.

The HqD enzyme activity products were subjected to organic solvent extraction using equal volume of ethyl acetate thrice in neutral, acidic and alkaline pH and evaporated to dryness using a Rotavapour (Buchi, Switzerland).

The resulting double-stranded cDNA was purified using equal volume of phenol : chloroform : isoamyl alcohol (25 : 25 : 1).

Exactly similar procedure was performed to control animals using equal volume of normal saline instead of AA solution.

Reverse transcription was carried out using equal volume of DNA-free RNA from each fraction with the M-MLV reverse transcriptase and the oligo-dT primer (Invitrogen).

After centrifugation at 8000 rpm, the pellet was discarded and the water layer was partitioned by using equal volume of ethyl acetate twice.