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Inhibition of viral replication by icIgA antibodies has only been observed with in vitro studies using epithelial cell lines in transwell cultures.
A gentamicin protection assay of Salmonella typhimurium was performed using epithelial cell lines that are engineered genetically to overexpress or lack mouse CHI3L1.
Many studies using epithelial cell lines have shown that paracellular hyperpermeability is caused by ATP depletion [ 28- 30].
In vitro studies using epithelial cell lines (A549) have demonstrated that exposure to particulate matter (PM) leads to increased apoptosis [ 22– 22].
The studies described herein were carried out using epithelial cell lines as well as a murine model of acute otitis media (OM).
Several studies have addressed the EV entry process using epithelial cell lines and human monocyte-derived dendritic cells (DCs) with conflicting results (Ichihashi, 1996; Vanderplasschen et al, 1998a; Locker et al, 2000; Law et al, 2006; Roberts et al, 2009; Sandgren et al, 2010).
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Most of the models use epithelial cell lines although primary cells are occasionally used also [ 28].
To establish an in vitro system that mimics the infection state in an epithelium, we co-culture lymphocytes with a widely used epithelial cell line, Madin-Darby canine kidney (MDCK) cells.
Using the rat liver epithelial cell line WB-344, transfected with AP-1 binding AP-1 bindingciferase reporter gene, the ability of remeDNAtion products of andclors to induce aP-1 activity was determined.
Using mammary epithelial cell lines (MCF10A, MCF12A, MCF7 and MCF7/RT) as a model system, we determined the impact of TG2 expression on cell growth, cell survival, invasion, and differentiation.
Experiments using mammalian epithelial cell lines have elucidated biosynthetic and recycling pathways for apical and basolateral plasma membrane proteins, and have identified components that guide apical and basolateral proteins along these pathways [31], [32].
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