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The fused GST-HTH domain of EcBirA (pGEN1) was subjected to biotinylation using enzymatic concentration of 100 nM MtBPL/EcBirA.
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Plasma lactate concentration was measured using enzymatic colorimetric method (Roche Diagnostics GmbH, Mannheim, Germany) at the time of each StO2 measurement.
The concentrations of metabolites were measured using enzymatic assays or HPLC.
Plasma lipid concentrations were measured using enzymatic reagent kit.
Glucose and ethanol extracellular concentrations were measured using enzymatic kits (rBiopharm, Darmstadt, Germany).
Plasma glucose concentrations were determined using enzymatic methods (QUICKAUTONEO GLU-HK, Shino-Test Co, Kanagawa, Japan).
The plasma concentrations of TAG and NEFA were measured using enzymatic reagent kits from Merck Eurolab (ref. 113009990314) and from Wako Chemicals (ref. RD291001200R), respectively.
After hydrolysis, monomeric glucose and xylose concentrations of the liquid samples were determined colorimetrically using enzymatic assay kits, as described previously by Gao et al.[ 44].
The present work aimed at the extraction and concentration of β-carotene from crude and refined buriti oils using enzymatic hydrolysis as a process strategy.
Serum concentration of total cholesterol (TC), triglycerides (TG), and high density lipoprotein cholesterol (HDL-C) were determined using enzymatic Infinity kits (Thermo Fisher Scientific Australia Pty Ltd ,Vic, Australia).
Bile and liver concentrations of cholesterol, bile salts and PC were also determined using enzymatic kits from Wako Chemicals.
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