Extracellular glucose and ethanol concentrations were determined enzymatically using enzymatic analysis kits (Sigma) as described by the manufacturer.
Glucose, lactate and glycerol were measured using enzymatic analysis kits from Sigma.
The organic acids (D/L-lactic acid and acetic acid) and ethanol contents of the samples were analysed spectrophotometrically using enzymatic analysis kits from Boehringer Mannheim according to the manufacturer instructions (R-Biopharm AG, Darmstadt, Germany).
To hydrolyze the substrate to fermentable sugar in the process, some desirable commercial enzyme reagents were selected based on regression analysis using enzymatic activities, and then, mixing ratio of them was optimized the design of experiments (DOE) with response surface method (RS M.
We performed a comprehensive analysis of external and internal exoDNA using enzymatic methods specific for dsDNA, and physical studies by imaging exoDNA using AFM.
Further compositional analysis was performed using enzymatic oligosaccharide mass spectrometry.
Plasma analysis for the lipid profile (using enzymatic test), fasting glucose (using glucose oxidase test), and insulin (using ELISA test) was done in laboratories of Isfahan Cardiovascular Research Institute.
Commercially available kits were used for plasma analysis and measurement of: glucose using an enzymatic-colorimetric kit (Sigma Chemical Co., St . Louis MO, kit #315 100) followed by spectrophometry; cholesterol using enzymatic kits (Sigma Chemical Co., St . Louis MO, kit #402 20) and triglycerides (Sigma Chemical Co., St . Louis MO, kit #344 20).
Total cholesterol and triglyceride were measured using enzymatic methods.
Serum cholesterol and lipoprotein levels were measured using enzymatic assays.
