The angiogenesis was determined by the forming of tube-like structures resembling capillaries using endothelial cell line (EA hy9.26).
We studied the process of leukocyte adhesion using endothelial cell line EA.hy926 and neutrophils progenitor cell line HL-60 labeled with BCECF-AM.
To investigate the role of endogenous αB-crystallin in inflammatory activation of endothelial cells, we used endothelial cell lines established from myocardial microvascular endothelial cells isolated from 129S6 wild type mice (MyEnd wt) or αB-crystallin-deficient (cryab - /- ) mice (MyEnd cryab - /-) [ 2].
To counteract the lack of pericytes, the tri-culture has been developed using endothelial cells, pericyte and astrocytes cell lines.
Several models have been described using immortalized endothelial cell lines such as mouse brain endothelial cells bEnd.3 [29], rat brain endothelial cells RBE4 [30,31], and hCMEC/D3 human endothelial cells [32].
Our studies, using human endothelial cell lines have shown that mobile phone radiation induces statistically significant changes in the expression of several tens of proteins [ 6] and that the response of cell might be proteome-dependent [ 7].
Examples of widely used brain endothelial cell lines described in the literature include the immortalized hCMEC/D3 human cell line [ 41], the rat RBE4 cell line [ 42] and the mouse bEnd.3 cell line [ 43].
Using the endothelial cell line EA.hy926, Grimm et al. [ 43] showed the formation of 3D tubular structures in clinorotation.
In this study, using human melanoma and endothelial cell lines, we have observed that treatment with a clinically used form of pegylated IFN- α treatment in combination with the chemotherapeutic agent, vinblastine (VBL), induces cell death via IRF-1-mediated signaling in melanoma cells and concurrently induces premature senescence in endothelial cells.
This is consistent with the findings of two in vitro studies that used human brain microvascular endothelial cell lines (HBMEC [ 48, 49] and hCMEC/D3 [ 50] to test whether cytoaherance to ECs is important for endothelial activation.
