Sentence examples for using double volumes of from inspiring English sources

Bone marrow samples were processed with a similar protocol, but using double volumes of buffer AL and ethanol.

A modified version of the Affymetrix GeneChip 2-cycle target labelling in vitro transcription (IVT) protocol was used, incorporating double volumes of polyA controls and reagents in the first round cRNA synthesis stage to increase the yield.

Silica extractions were performed using QIAamp DNA micro extraction kits (Qiagen) following the manufacturer's guidelines, although using double the suggested volumes of all reagents prior to the wash stages (proteinase k, buffers AL and ATL, 100% Ethanol).

Volume of the HRP-containing compartments was quantitated by point counting using double lattice grids to relate points over HRP-labeled profiles to the cytoplasmic volume using standard methods [46].

A flow chamber with a volume of about 5 µl was built by assembling two glass coverslips on top of each other using double sticky tape (Tesa).

The use of a double volume of solution R for the first treatment was adopted to overcome the immediate buffering power of the soil, to avoid its deactivating effect and to reduce the chemical reagents usage.

In this study, a new approach of using double-layer pigmented coating with different volume fractions of CuO submicron particles is proposed to achieve better performance.

Fluorescently labelled glycans were digested in 50 mM sodium acetate (pH 5.5) at 37°C overnight using a panel of enzymes with each digestion reaction brought to a final volume of 10 μl using double-distilled water.

We used the same stripping parameters as in the buffer, except for double volume of 200 μL and preconcentration time of 600 s.

The reaction was quenched using five volumes of ice-cold double-distilled water.

Double-stranded DNA was purified using 1.8 volumes of AMPure XP beads (Agencourt) and eluted in water.