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Absolute numbers of transcripts per embryo given at the 60-cell stage are averages of the results of two independent experiments using distinct batches of cDNA.
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The impact of using different CSCB preparations for enzyme assays was assessed in three distinct batches of this substrate with the same sample of the commercial T. viride cellulase.
However, if the user suspects that batch effects could confound the results, in particular if two single and distinct batches of samples are loaded as pool 'A' and 'B', respectively, it could be useful to attempt removing batch effects from the raw data using one of the existing tools [ 30] prior to importing data in TRAM.
For the in vitro studies, all experiments were performed with two distinct batches of ECM in at least duplicate.
This probably reflects the fact that the cellulose content of distinct batches of sugarcane bagasse does not vary very strongly [ 10].
The effect of 100 µM ALA on parasite growth varied slightly between experiments, possibly due to differences between distinct batches of donated erythrocytes.
(1) Researchers found that a distinct batch of cells lit up when the monkees performed or even observed specific movements.
This normalization procedure uses distinct biological replicates to help mitigate non-specific transcriptional responses associated with many perturbations as well as experimental batch effects.
This result is an average of three experiments using different batches of keratinocytes.
Inter-assay variation (batch to batch) was determined by measurement of the same samples in the same run using different batches of coupled beads.
The experiments were repeated using three different batches of cells.
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