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Epidermal thickness was measured serially using digital images of histology slides stained with hematoxylin and eosin.
Distances from vascular stroma to regions of NESP55 immunreactivity were measured using digital images of NESP55-stained tumor sections and an index scale.
Disruptions in microtubule immunoreactivity were quantified for size and luminosity using digital images of MAP-2 immunoreactivity and Image J. Luminosity was calculated as the average of three measurements within a 75×75 µm box randomly placed within the damaged area.
We measured quantitative data using ImageQuant GE Healthcaree, Piscataway, NJ) using digital images of scanned films.
Kanamycin resistant bacteria were enumerated using digital images of the plates, which were acquired with a Q-Counter (Spiral Biotech, Norwood MA)) after 28 and 48 h of incubation.
Using digital images of the stained material (objective lens magnification 20×), thickness of the membranes and percentage of fibres of the whole membrane area were determined by light microscopy (Olympus CX41 with digital camera DP20, Olympus, Hamburg, Germany) using the image analysis software ImageJ v1.44 (US National Institutes of Health, Bethesda, MD, USA).
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The move follows an article Thursday by The New York Times and ProPublica about an exclusive deal that Memorial Sloan Kettering made with an artificial intelligence start-up to use digital images of 25 million tissue slides analyzed over decades.
The device, called MelaFind, uses "digital images of skin growths and uses a computer to analyze them for signs of cancer, offering a sort of second opinion to doctors," according to The Associated Press.
Dr. Vunjak-Novakovic, Dr. Warren L. Grayson and other members of the team used digital images of the joint to guide a machine that carved a three-dimensional replica, called a scaffold, from cleansed bone material.
The system, based on multivariate and multiresolution texture analysis, uses digital images of the bulk material to extract quantitative information on the particle size ranges appearing in each image and on their weight proportion independently of the shape of the particle distribution (mono- or multi-modal).
In some cases, we used digital images of 10 mm-thick histological sections to carry out three-dimensional reconstructions using the software AMIRA 4.1.2 (Mercury Computer Systems).
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