Exact(14)
The hybridization probe was detected with an alkaline phosphatase-conjugated anti-DIG antibody using DIG nucleic acid detection kit (Roche Diagnostics).
Northern blot was probed with dig-labeled antisense probe, and visualized using DIG luminescent detection kit for nucleic acids (Roche).
Human IGF-I specific bands were detected by denatured DIG-labeled hIGF-I probe prepared by PCR using DIG DNA labeling Kit (Roche).
Hybridizations and washes were carried out using DIG hybridization buffer according to the manufacturer's directions (Roche).
Alike to the latter four steps of the previous scenario, we iterate the list of domains and perform DNSSEC validation using Dig and a DNSSEC-capable resolver.
Probe was prepared using PCR product of TvGST gene using DIG labeling kit (Roche Biochemicals, Germany).
Similar(46)
For in situ hybridization experiments, DIG-labeled (Roche) antisense RNA was transcribed from a linearized fragment using DIG-labeled dNTPs.
DNA hybridization probes were amplified with primers GL726/GL727 and then labeled with digoxigenin-11-dUTP using DIG-High prime according to the instructions (11745832910, Roche, Shanghai, China).
Double ISH was carried out using DIG- and FITC-labeled riboprobes as previously described [23].
The 56-bp dsDNA (5'-GATCAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCC-3') was end labeled with using DIG-11-ddUTP (Roche) [45].
The expression detected with Pngl antisense probes is specific as no signal was detected using DIG-labeled RNA probes prepared against the sense strand of cDNA (Fig. 3E).
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