Sentence examples for using different primers and from inspiring English sources

A pyrosequencing approach to determining beta-defensin copy number has been described previously [42], using different primers and an alternative paralogue on chromosome 5, giving results that differed considerably from MLPA and previously published estimates of the same samples [50].

Any positive sample was tested again by using different primers and real-time PCR technology.

We also showed previously using different primers and PCR conditions that G0S2 is predominantly expressed by monocytes.

As this finding suggests a misclassification bias, they retested a random of their samples (5%) using different primers and restriction enzymes.

The expression levels of the promoter-specific transcript variants were assessed using different primers and assay-specific standard curves (i.e. different qPCR efficiencies), and the data are therefore not directly comparable.

Despite strong sequence conservation between predicted orthologs and various attempts using different primer and PCR conditions, we were unable to verify the 5' coding regions of AmNLG2.

We therefore used different primer and probe combinations to discriminate between EVI1, MDS1 EVI1, and MDS1 and quantified their transcript levels in 18 of the HCC cell lines using quantitative TaqMan PCR analysis.

Positive and negative control binding regions were established using different primers sets, and data was expressed as percentage of input for each region.

Further studies using different primers and/or specific tissues and developmental stages are needed to substantiate whether or not an AHR-like molecule exists in E. fetida.

Multiple PCR attempts combined with using different primers resulted in success for 3 of these 4 genera.

This was verified by variant rates of failed detections observed for the same phylum dataset using different primers.