Sentence examples for using different primer sets from inspiring English sources

It may be the case that the cassettes present in the Tar Ponds will prove to be associated with class 1 integrase genes as no other types of integron integrase were found by us despite using different primer sets.

This pattern is strongly reflected in high correlation between the number of reads from two sources of DNA using different primer sets and leads us to deduce that primers bind to each species in the mixture with the same efficiency between two different sources of DNA.

Finally, only class 1 integrase genes were amplified in this study despite using different primer sets, and it may be that the cassettes present in the Tar Ponds will prove to be associated with class 1 integrase genes.

Several potential 5 S locus transcripts were examined by RT-PCR using different primer sets.

Subsequently, DNA was amplified with PCR using different primer sets (Table S2).

qPCR was performed as previously described [ 50] using different primer sets (Additional file 5: Table S1).

cDNA was amplified using different primers sets, which amplified different regions of all sequences from exon 11 to exon 20.

Positive and negative control binding regions were established using different primers sets, and data was expressed as percentage of input for each region.

To further evaluate whether the sustained activation of PDGF βR in EqS cell lines might be caused by activating mutations in the transmembrane and/or cytosolic domain, cDNAs of exons 11 20 of PDGF βR were amplified by RT-PCR using different primers sets followed by sequencing.

We believe this is justified with regards to sensitivity and specificity as the PCRs use different primer sets and target regions.

Genetic libraries of the plastid 16S rRNA gene were constructed on picoeukaryote populations sorted by flow cytometry, using two different primer sets, OXY107F/OXY1313R commonly used to amplify oxygenic organisms, and PLA491F/OXY1313R, biased towards plastids of marine algae.