Sentence examples for using different primer mixes from inspiring English sources

We performed a stepwise analysis using different primer mixes for different levels in the phylogeny (Supplementary Information, Table S2).

One approach employed in our laboratory is to first use the dual labeling method to fine-tune the dyes associated with different primer mixes before ordering the prelabeled forward primers direct from a manufacturer for the full analysis; this is particularly effective for large-scale studies involving hundreds of individuals.

Four different primer mixes (MP2, MP3, MP5, and MP9 see Table 1) each amplifying two, three or four microsatellite loci were used.

Nested PCR was performed using different combinations of the gene-specific primers and the RACE primer.

These results yet clearly demonstrate the importance to use four different DRT primer mixes.

gDNA template (previously prepared) was used for comparison using the same primer mix as the cDNA reaction.

Immunoglobulin cDNA was synthesized using a primer mix and variable regions amplified using specific primers.

Then 1 μg of RNA was reverse transcribed into cDNA for analysis by qPCR using primers specific to the indicated locus (using QuantiTect Primer Mix from QIAGEN).

We used 5 different primer pairs.

Briefly, 100 ng of total RNA was reverse transcribed using the RT primer mix provided by the kit, which contains one RT primer for each of the 35 different apoptosis-related genes and four control genes in a single multiplex reaction.

For each method, four different DRT primer mixes (A-D) have been used.