Sentence examples for using different primer combinations from inspiring English sources

The alternative chromosomal arrangements were then determined by direct PCR using different primer combinations, with genomic DNA samples from strains H99 and JEC21 serving as positive/negative controls (depending on the primer combination).

The orientations were distinguished by PCR using different primer combinations (see below).

No amplification could be obtained with MtCBF13-specific primers from both accessions despite using different primer combinations and PCR conditions.

Furthermore, several multiplex PCRs have been described for identification of Brucella partly at the biovar level using different primer combinations.

The putative transgenic embryos/plants were PCR-screened for the presence of the complete T-DNA using different primer combinations (Table  5).

In total, approximately 3000 transcript-derived fragments were monitored ranging from 50 to 600 bp in size using different primer combinations (Additional file 1).

In the case of archaea, we used different primer combinations to recover a maximal variety of archaeal genes.

In a preliminary experiment that used different primer combinations, the combination EcoRI-AC + MseI-A adaptors gave excellent results, yielding readable profiles with well-separated bands.

Further extensive PCR using different primer pair combinations (of those shown in Fig. 2C) confirmed that no full-length transcript of AHK5 was present in the mutant lines (Data S1).

We therefore used different primer and probe combinations to discriminate between EVI1, MDS1 EVI1, and MDS1 and quantified their transcript levels in 18 of the HCC cell lines using quantitative TaqMan PCR analysis.

Using ten different primer combinations, a total of 784 bands were scored, 776 were polymorphic representing 98.9% of the total number of bands (Table 4).