The MGMT assay was performed three times for each extract, using different extract volumes and the amount of [H]methyl groups transferred from substrate calf thymus DNA (Sigma) to protein, under conditions of excess substrate, was quantified by liquid scintillation counting.
The quantification of eight BA's was carried out in different extracts prepared by using different extraction techniques.
We have already worked for the synthesis of different functionalized Au and Ag nanoparticles using different plant extracts of clove and guava (Raghunandan et al. 2009; Raghunandan et al. 2010a; Raghunandan et al. 2010b).
Biofunctionalized gold and silver nanoparticles synthesized using different plant extracts of guava and clove in vitro anti-cancer efficacy against four different cancer cell lines human colorectal adenocarcinoma, human kidney, human chronic myelogenous, leukemia, bone marrow, and human cervix have been studied and reported.
The 50%% inhibitory concentration (IC50) was evaluated using different concentrations of cyanobacterial extracts equivalent to 1 µg to 10 mg dry weight of respective strains.
Therefore, to extract all compounds it is important to extract using different solvents of varying polarity to cover the polarity range.
This could be related to the use of different extracts of TwHF, both of which were poorly characterized.
13 This information has, in fact, led two companies (Phoenix Biotechnology, Inc and Nerium Biotechnology, Inc) to explore the use of different extracts of N. oleander for anticancer activity.
Figure 2 Synthesis of SNPs using different leaf extract concentrations.
The next step was to determine the nature of the inhibition exerted by the aqueous extract of A. macroprophyllata, then a kinetic analysis of the inhibition of enzyme activity using different amounts of aqueous extract or acarbose was conducted.
