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A standard addition method for a calibration based on four-way data was applied using different dilutions of the extract from iceberg lettuce as a fourth way that provided the enough variation of the matrix to carry out the four-way analysis.
A standard curve was obtained in untreated cells using different dilutions of Herceptin-Alexa Fluor 680 in lysis buffer.
Comparative assessment of overall reactivity of secretory material with the mAbs was made by ELISA, as described above, by using different dilutions of fungal secretions as plastic-bound antigens.
Post-stimulus aggregation was assayed using different dilutions of apple cider vinegar.
The lipoxygenase (LOX) inhibition assay was conducted by using different dilutions of the PR by following previously described method [ 25].
For calculations, a standard curve was determined for each gene using different dilutions of the cDNA products.
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Sudár et al. [61] used different dilutions of the eluates for ecotoxicity testing and established dose response curves.
Each extract was assayed in 4 6 replicates using different dilutions.
The method of the Japanese Society of Chemotherapy used different dilution and medium from the CLSI method.
Initially, these multiplex protocols were tested using different serial dilutions of genomic DNA extracted from peripheral blood (from 100 ng/mL to 5 pg/mL).
Lysates were purified using acetone precipitation of proteins, and supernatants from each sample were quantified using two different dilutions of each.
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