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Results shown in each histogram are representative of at least three experiments carried out using different cell populations.
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In this study we use different cell populations cultured directly from primary-derived patient material (so-called cancer stem cells, as well as freshly differentiated cells) to investigate the combination of a pharmacological inhibitor of PI3K, GDC-0941, and clinically relevant doses of radiation.
Ince et al. used different cell culture conditions to enrich for either luminal-like or myoepithelial-like human breast cell populations [ 14].
The amplitude, opacity, and phase information can be used to discriminate different cell populations without the use of cell markers [ 61].
FlowJo software (Ashland, OR) was used to quantify different cell populations in flow cytometry experiments.
Multiple independent transgenes can be easily introduced in the same cell and cell type specific promoters can be used to target different cell populations.
For this purpose, cell surface antigens are used to characterize different cell populations.
This is the model Liu et al., 2012 proposed by labeling different cell populations using mosaic analysis with double markers (MADM) in mice.
The use of the database enabled us to identify different cell populations among cell mixtures.
These results are different from those of Apps et al. 9 with regards to the β2m association of HLA-G dimer in placenta, and might be a consequence of the different cell populations used in each study.
Data analyses of flow cytometry data were carried out using Prism version 3.0 (GraphPad Software) using the unpaired t-test to compare different cell populations.
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