This was the same as the highest value published for M. alpina ME-1 (Jin et al. 2008) and was much higher than the 1.50 g/L day−1 reported for M. alpina DSA-12 using conventional protocols (Hwang et al. 2005).
In preparing small noncoding RNAs using conventional protocols, we discovered that these protocols also enrich for small DNA pieces.
Intracellular protein extracts were prepared using conventional protocols (Millar et al., 1992).
RT-PCR analysis showed the full expression of all osteogenic markers typical of late stage osteoblasts after 30 days of colture, while the same expression pattern is showed after 50 days from withdrawal by cells obtained using conventional protocols.
Tissues were formalin-fixed, treated for 1 hr in 98% formic acid, and embedded in paraffin using conventional protocol.
To evaluate performance of the new chip-based system, median lethal concentrations (LC50) of a panel of reference toxicants obtained on this system were compared with those from tests using conventional static protocols, and were not significantly different.
While microneedle technology to collect ISF from the skin has advanced considerably, there has been little attention paid to collection of analytes from microneedle patches for analysis using conventional biodiagnostic protocols and equipment.
To confirm the authenticity and accuracy of the GS-FLX generated data, a small number of mtDNA and nuclear DNA sequences were targeted using conventional PCR protocols, following a two-steps protocol [41] and 50 degrees of annealing temperature.
Thereafter, tissue sections were washed in PBS 3 × 3-minute intervals and incubated for 90 seconds in 0.05% 3,3-diaminobenzadine and 0.015% H2O2 (Sigma-Aldrich, St . Louis MO) in water, then washed in tap water, counterstained with hematoxylin and eosin (H&E; Thermo Fisher, Waltham, MA) using a conventional protocol, and cover-slipped using Surgipath Micromount medium (Leica, Germany).
All patients were treated in a private practice where asking for radiographs at T1-T2-T3 is conormaled normal part of the orthodontic treatment; moreover, all patients were treated using a "conventional protocol" and any experimental procedure was used.
