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We conclude that placebo analgesia in rats is not particularly robust and that it is difficult to achieve using conventional procedures and proper experimental design.
Current protocols for the purification of both native and polyHis-tagged or glutathione-S-transferase (GST -tagged EF-Tu proteins and their variants usinGST -taggednal procedures and the Ni-NTA-Agarose or Glutathione Seproteinsand presentheir
All constructs were generated using conventional procedures and verified by DNA sequencing.
All analyses of descriptive data were performed using conventional procedures and the results are expressed as means and standard deviations (SD).
Nerve conduction studies were performed in the bilateral median nerves using conventional procedures and an EMG Machine MEB-23000, Nihon-koden).
Plasmid DNAs were extracted and BigDye Terminator (ABI) cycle sequenced on ABI 3730 sequencers using conventional procedures and the following primers: 5'-T18-3', M13 forward (5'-GTAAAACGACGGCCAGT-3'), M13 reverse (5'-AACAGCTATGACCAT-3' or 5'-CAGGAAACAGCTATGAC-3'), and SP6WAN (5'-ATTTAGGTGACACTATAG-3') for 3' end sequencing of Evrogen libraries.
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The procedure gives structural information of the column, and therefore iterative computation encountered in the design using conventional procedure and commercial packages can be eliminated.
The pieces of ESCC tissue were processed using conventional procedures for paraffin embedding, and cut into 5-μm thickness.
Composite T-scores are calculated for multiple goals and change over time is quantified using change scores and using conventional procedures recommended in literature [ 19].
Oesophageal squamous cell carcinoma tissue specimens were cut open longitudinally, fixed with formalin, and then processed using conventional procedures.
The study was therefore designed to tag and release half the fish into the ocean as described in the methods section and the other half were released into the river using conventional procedures.
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