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The large number of NRs (48 in humans) makes it time-consuming and difficult to discover such a network using conventional molecular techniques alone (Bookout et al, 2006; Yang et al, 2006).
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Prior to this the focus of each laboratory was limited to one or a few genes selected by hypotheses generated from the available literature or laboratory experience, using conventional molecular biological techniques.
This is difficult to achieve using existing technologies, which typically use conventional molecular biology techniques to fuse gfp to the 5' or 3' end of the target gene [14] [16].
While identification of P. aeruginosa from a positive blood culture (BC) takes at least 24 h using conventional techniques, molecular identification directly from positive BCs could be an interesting alternative leading to a rapid and accurate species-level identification with subsequent adequate empirical treatment.
Expression patterns were validated by confirming mRNA expression patterns with conventional molecular techniques.
Therefore, before starting any improvement program using conventional or molecular breeding techniques, understanding gene function and the genome scale of Jatropha are prerequisites.
Although some cotton cultivars resistant to V. dahliae have been generated using conventional breeding techniques, the molecular mechanisms of disease resistance in cotton are still unclear.
All isolates were identified using conventional biochemical techniques [ 9] before molecular identification by the GenoType Mycobacterium CM/AS assay.
When we proceed to treat patients with these procedures, we must take into account that myoglobin has a molecular mass of 17 kDa and is poorly removed from circulation using conventional extracorporeal techniques.
Such metal gratings can be created using conventional fabrication techniques.
Another is to start creating cavities that are impossible to manufacture using conventional machining techniques.
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