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Using massively parallel sequencing data from degraded body fluids, we designed primers to amplify transcript regions of high read coverage, hence, higher stability, and compared these with primers designed using conventional methodology.
This is new and valuable information that until now could not be obtained using conventional methodology such as extraction or autoradiography.
These results were very similar to the well-documented expression profile of C/EBPβ using conventional methodology and consistent with its role in regulating the expression of C/EBPα and PPARγ, two master regulators of adipogenesis that are induced ∼48 hrs into differentiation [25], [26].
Prevalence and associated 95% confidence intervals were calculated using conventional methodology [ 21].
Dvl2 floxed allele was generated in the UNC Neuroscience Center Molecular Neuroscience Core using conventional methodology (Ohata et al., 2014).
This allowed the selection of the best production host, growth temperature, time for induction and inducer concentration by using conventional methodology with changing one variable at a time.
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Three decades of intensive research have yielded four established AD genes (APP, PSEN1, PSEN2, APOE), and hundreds of potential susceptibility loci, none of which has been unequivocally shown to modify disease risk using conventional methodologies.
Recent advancements in the neuroscience and engineering of neuroprosthetics are providing a blueprint for how new co-adaptive designs based on reinforcement learning change the nature of a user's ability to accomplish tasks that were not possible using conventional methodologies.
DNA was extracted from samples using conventional methodologies and quantified using PicoGreen (Invitrogen).
DNA was extracted from EDTA venous blood samples using conventional methodologies and quantified using PicoGreen (Invitrogen, Paisley, UK).
DNA was extracted from samples using conventional methodologies and quantified using PicoGreen (Invitrogen Corp., Carlsbad, CA, USA).
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